[Histonet] electrical charge slides

[Gayle Callis]

Bill,

Would you explain what is used for coating that contains calcium?  Does 
Silane contain calcium?  Poly L lysine?  If there another coating we are 
not aware of, the one containing calcium?  I have never heard this theory 
before and am fascinated by your comments.

- At 08:54 PM 8/5/2004, you wrote:

>Dana,
>
>There was heaps written about this on Histonet previously, look in the
>archives.
>We found that the problem was associated with the Plus slides when used in
>conjunction with an EDTA buffer as the material used to coat the slides is a
>calcium salt and the EDTA was affecting the coating by chelating the
>calcium!!!
>
>All the best
>Bill Sinai
>Laboratory Manager
>Tissue Pathology, ICPMR
>Westmead NSW 2145
>Australia
>Ph 02 9845 7774
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
>DDittus787 <@t> aol.com
>Sent: Friday, 06 August 2004 12:10 AM
>To: histonet <@t> pathology.swmed.edu
>Subject: [Histonet] electrical charge slides
>
>
>To my colleagues:
>
>I have a question.Is anyone out there having occasional staining(orlack of
>staining on IHc slides?) I was told that the charge or the way the slides
>are charged can have (pardon the pun) a negative effect. Does anyone know
>/experience or have the scientific data for this?
>                                  Dana
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)