[Histonet] ID-2

[Kimberle M. Jacobs]

Has anyone used the antibody to ID-2?  We purchased it from Santa Cruz 
Biotech.  They had some recommendations for western blot, but none for 
immunohistochemistry.
Our goal is simply to label the superficial layers of ferret visual 
cortex.  But on our first try using 1:50, 1:100, and 1:200 primary 
dilutions - it didn't work at all.
We were wondering if we need to do some antigen revealing step.  Or perhaps 
it will not be found this caudal?

Anyone know of any antibodies that label a specific layer in visual 
cortex?  I've heard that the otx1 antibody is not working well right 
now.  We are going to get RORbeta to attempt to stain layer IV, but again - 
no specific protocol for it.

Thanks very much for any help!

Kimberle M. Jacobs, Ph.D.
Assistant Professor
Department of Anatomy and Neurobiology
Virginia Commonwealth University
P.O. Box 980709
Richmond, VA  23298-0709
(804) 827-2135
FAX: (804) 828-9477
kmjacobs <@t> vcu.edu