[Histonet] Spleen autofluorescence

[Sharon Cooperman]

I have had pretty good luck quenching autofluorescence in FFPE spleen 
with sodium borohydride 0.5 mg/ml in PBS pH 8.0 x 10 minutes x 3 
changes.  It works better than H2O2 for me.

Sharon

>Hi histonetters,
>
>Apologies if my query is really basic but I am wanting to do immunofluorescent
>antibody staining on mouse spleen sections (paraffin-embedded) and am seeing a
>lot of autofluorescence, which I presume is due to platelets. Is there any way
>around this problem?
>
>Helen Newbery,
>Medical Genetics Section,
>Molecular Medicine Centre,
>Western General Hospital,
>Edinburgh,
>EH4 2XU.
>0131 651 1047
>
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-- 
Sharon Cooperman       	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892