[Histonet] XIAP

Garry Ashton GAshton <@t> PICR.man.ac.uk
Tue Aug 3 10:27:30 CDT 2004

Dear Histonetters,
I'm having problems using the above antibody from various sources.
I'm getting non-specific staining on my knock out mice specimens, with
no true positive staining seen in any of the WT mice.
Firstly has anybody out there used a XIAP antibody on FFPE sections, and
if so which one of the several available, (or even on frozens).
Secondly what is the best method of preventing non specific staining of
the antibody. In the past I've made the antibody in 1% casein or normal
serum from which the secondary is made, after applying a 10% solution to
the sections prior to the antibody incubation.
I've also tried various retrieval methods.
Many thanks in advance for any much needed help.

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