[Histonet] Secondary Antibodies dilutions

[Gayle Callis]

A rule of thumb is to use 2 - 10 ug/ml, do the calculation from 
concentration of antibody found as mg/ml in the specification sheets.  Some 
people even use a lesser concentration - we have done dilution panels on 
our secondaries at times to determine the optimal concentration needed in 
ug/ml.   We tend to average around 1:250 dilution on 0.5mg/ml 
concentration.  Specification sheets usually give the recommended dilution 
for immunohistochemistry usage.

At 05:41 PM 8/2/2004, you wrote:
>I wanted to know if some of you uses the following  Antibodies  , and to
>that dilution uses them in immunohistochemistry.
>
>Biotin-Goat anti-Rabbit IgG (Zymed 65-6140
>
>Biotin-Goat anti-Mouse (Chemicon AP181B)
>
>Thanks
>
>Dr. Hugo H. Ortega (DMV, PhD)
>
>Departament of Cellular Biology
>
>Faculty of Veterinary Sciences
>
>Universidad Nacional del Litoral
>
>R.P. Kreder 2805 - Esperanza (3080)
>
>Santa Fe - ARGENTINA
>
>Tel. (54)3496-420639
>
>Fax. (54)3496-426304
>
>  <http://fcv.unl.edu.ar/histolog/> http://fcv.unl.edu.ar/histolog/
>
>  <http://fcv.unl.edu.ar/bioterio/> http://fcv.unl.edu.ar/bioterio/
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)