[Histonet] Re: Zinc formalin and 10% NBF
Marshall Terry Dr,
Terry.Marshall <@t> rothgen.nhs.uk
Wed Apr 28 10:09:23 CDT 2004
"It was amazing to see how firm and grayish the tissue was as compared to
the NBF fixed tissue, within even an hour of fixation time."
I too am eager to learn what formulation you are using. The first time I tried zinc, (in 1971), I used about 8% (because I had no idea what strength to use - just trying a poor man's mercury), and the tissue went the colour and consistency of concrete within half an hour. In fact, it only takes homeopathic doses of zinc to do the trick.
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Rotherham General Hospital
terry.marshall <@t> rothgen.nhs.uk
From: Johnson, Teri [mailto:TJJ <@t> Stowers-Institute.org]
Sent: 27 April 2004 19:15
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Re: Zinc formalin and 10% NBF
In working through our processing schedule dilemma, we fixed some
pancreas in NBF (overnight), and fixed some in Zinc Formalin for 3
hours, followed by water wash and storage in 70% ethanol. We found that
using a variety of processing schedules, the NBF fixed pancreas looked
good with routine 30 min/station (with 2 hours total time in paraffin),
all the way down to 10 min/station (with 80 min total time in paraffin).
The Zinc Formalin fixed pancreas looks dry and brittle still, even with
the short processing schedule.
We will try a hand-processing schedule for these to see if we can get
the processing perfect for the ZnF pancreas. Our answer to this problem
could be that we need to limit the amount of time it is in fixative.
Gayle, how long were the kidney biopsies fixed before processing?
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj <@t> stowers-institute.org
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