[Histonet] RE: Cryojane cutting and LCM

Wright, Barbara (SPRI 2) barbara.wright2 <@t> dnax.org
Tue Apr 27 08:34:42 CDT 2004


I believe you need to use 1/2x slides for cryosjane sectioning (instead of
the 1x) if you are going to use them for LCM.


-----Original Message-----
From: Lori Wilson [mailto:WilsonL <@t> jwci.org] 
Sent: Monday, April 26, 2004 6:20 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Cryojane cutting and LCM

Has anyone used the cryojane with OCT frozen sections to perform LCM?  We
are using it for protein extraction and mass spectrometry analysis.  I am
having incomplete microdissection and there is an odd background staining.
Does anyone have experience with this?  



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