[Histonet] Proteoglycan - decalcification

[balajimr <@t> drreddys.com]

Dear Callis,

Thank you very much for your replies to my earlier mails. This is with 
regard to Saf/FG staining of cartilage. I want to demonstrate 
proteoglycans in cartilage of femur joint, tarsal joint and ankle joint. I 
am in the process of standardization of the same. I have taken a trial 
tissue of joints from Rat and I have used 20% EDTA  for decalcification. I 
read somewhere that it affects the proteoglycan staining. Is it true? If 
so could you please suggest a good decalcifier for the same.Also let me 
know how long I can fix these joints for good fixation in NBF. Thanks in 
advance.

Dr. Balaji
Dept. Pre clinical safety evaluation,
Discovery research, 
Dr. Reddys Laboratories ltd. Bollaram Road, 
Miyapur, Hyderabad, 500 049
 Andhra Pradseh, INDIA

Tel: 23045439 - Ext.432.