[Histonet] re Ag retrieval
carl.hobbs <@t> kcl.ac.uk
Tue Apr 20 10:34:06 CDT 2004
Thanks for that , Steve.
I must admit to the opposite lol. TRIS at pH 10 gives me better
morphological preservation than pH9 ( sections more inclined to fall off,
and much better/consistent preservation than at pH6..............(Nuclei
lose quite a bit of Haematein- reactivity equally)
Also, I have just seen some pictures of Chris van der Loos, clearly
demonstrating greatly increased disclosure of endog biotin at the higher pH
values. Again, the opposite to my experiences. Ok, ok...someone out there
must know what's going on?
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