[Histonet] IF on frozen sections

[Rena Fail]

We routinely embed skins in OCT and snap freeze in Liquid nitrogen
cooled isopentane
Rena Fail

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun
Lang
Sent: Monday, April 19, 2004 12:34 PM
To: Histonetliste
Subject: [Histonet] IF on frozen sections


A question about immunfluorescence on frozen skin sections:
We mount the skin with buffer on the sample-table to freeze it. After
sectioning we let the cuts airdry and do the IF (Immunglobulins). Is
there any reason, why we cannot use OCT for mounting? I think sectioning
would be easier. Perhaps someone can help me?

Lang Gudrun

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