[Histonet] Peeling

Christian Franci cfranci <@t> rigel.com
Mon Apr 19 16:39:40 CDT 2004


We've been routinely sending out blocks for sectioning (we're a bit short
handed at the moment) with out any problems until recently.
The slides I got back looked fine.  After HIER, though, the tissues on them
just kept peeling off as I was staining them....
So, I had them sent out again and the sections put on super charged slides.
Well, while the tissues didn't peel off as much, I still lost a fair
amount...
Is there any way to prevent this?
Any way to reduce peeling after HIER?
Or, am I *@#!! out of luck?
My samples represent too much work to watch them being lost.
Thanks in advance!
Cheers
C





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