[Histonet] fixation for frozen tissue

[Fred Underwood]

Hi Rita.

I find that washing the tissue in tap water after taking it out of the
formalin makes frozen sectioning easier.

Fred

>>> Rita Angel <RITA.ANGEL <@t> UC.EDU> 04/19/04 01:24PM >>>
Hi all,

I have several questions about fixing frozen tissue. In the past, we've

always frozen tissue fresh from the animal into frozen embedding media
and 
then immersing into liquid nitrogen.

I have an investigator that brought frozen tissue that he first fixed
in 
formalin, then froze. I'm unable to get sections because the tissue is

soft. It seems there is still moisture in the tissue, so I melted the
block 
& blotted off the tissue, then re-froze. I'm still not able to get
sections 
although it doesn't seem as mushy now. It still seems like the tissue
is 
wet.Does anyone have any suggestions?

He was also asking about a protocol for sucrose, and I was wondering if

anyone could get one to me? Also why do people use this procedure, and
when 
do you need to use it?

Thanks for all your help,

Rita Angel, HT


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