[Histonet] Microwave Processing

[Connie McManus]

Has anyone read the latest issue of the NSH's In Action??? Front page
story deals with this issue of microwaves domestic vs lab.  The NCCLS
has just come out with some guidelines dealing with this.  I suggest a
little visit to http://www.nccls.org and check out these guidelines.  
 
for what it's worth....
 
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805
 
"Where am I going and why am I in the handbasket???"
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bryan
Llewellyn
Sent: Friday, April 16, 2004 8:41 AM
To: Histonet
Subject: Re: [Histonet] Microwave Processing
 
I haven't used microwave fixation, but I do remember frozen section heat
fixation when I first started learning histology.  Perhaps saline at 65C
is
a modification of this?  We brought saline (or water) to the boil, took
it
off the heat and dropped a thin piece of fresh tissue into it for one
minute.  The tissue was then frozen on a clinical microtome with CO2 or
a
Peltier cooler and sectioned.  The morphology and staining was fine,
except
that eosin was pinker than expected.  It is important not to leave the
tissue too long in the hot saline or it cooked and it was ruined.  One
minute was about the maximum.  I've also tried fixing fresh tissue, in
cassettes, in NBF at 65C in an oven for an hour.  That works quite well
too,
at least as far as morphology and ordinary dye staining is concerned.
Of
course, a well sealed container is strongly advised.
 
Bryan Llewellyn
 
 
----- Original Message ----- 
From: "Marshall Terry Dr,Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
To: "kevin williams" <akwilliams75 <@t> hotmail.com>;
<histonet <@t> pathology.swmed.edu>
Sent: Friday, April 16, 2004 7:58 AM
Subject: RE: [Histonet] Microwave Processing
 
 
Sorry to but in - but on a related question - does anyone do microwave
fixation in saline as described by for instance the Melbourne group,
which
involves bringing up to 65C in saline?
Have tried it this week and the sections are even crummier than usual.
When I was in Tasmania, a local private lab. did it and their sections
were
fine.
 
Dr Terry L (why can't I get a good H&E) Marshall, B.A.(Law),
M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk
 
-----Original Message-----
From: kevin williams [mailto:akwilliams75 <@t> hotmail.com]
Sent: 16 April 2004 15:33
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Microwave Processing and Derm Shaves
 
 
 
   Is anyone out there doing any processing on derm shaves with
microwave
   processing? How is it and how are the results?
   Thanks.
 
   A. Kevin Williams
   Vermont Dermatopathology.
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References
 
   1. http://g.msn.com/8HMBENUS/2752??PS=
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