[Histonet] cutting frozen rat turbs,, a reply
gcallis <@t> montana.edu
Fri Apr 16 13:14:40 CDT 2004
I am presuming you mean UNDECALCIFIED rat turbinates? We do mouse
undecalcified turbinates, but need to sue the tape transfer system,
Cryojane from Instrumedics OR you crush delicate turbinate bone, do not get
a flat section that adheres to slide surface hence falling off.
It requires a d profile (some like c profile) tungsten carbide knife in
extremely sharp condition in order to cut decently through bone.
Without these tools, we get terrible sections.
At 09:09 AM 4/16/2004 -0600, you wrote:
>Has anyone out there cut frozen rat turbinates?? We are having trouble
>keeping them on the slides. Any pointers?
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