[Histonet] detach cells from a slide

Connie McManus convmcm <@t> cc.usu.edu
Wed Apr 14 16:55:10 CDT 2004

In cell culture, when cells need to be transferred to a new flask, a
0.25% trypsin for 15 minutes is used to detatch the cells from the flask
surface.  I don't know if that would work with blood cells, but you
could experiment to see. If you try it, I wouldn't let the cells soak as
long as 15 minutes.  I'd watch the solution for cell lift off and call
it good when I got most of them. 

just another wild and crazy notion...

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dolors
Sent: Wednesday, April 14, 2004 9:17 AM
Subject: [Histonet] detach cells from a slide

Any idea to detach cells from an hematologic slide?

They are dryed...stained with May Grünwald Giemsa...dehidrated, cleared
mounted with DPX.

The idea is to use them for a DNA extraction.

Thanks in advance

Dolors Fuster
Tècnic Especialista en Anatomia Patològica i Citologia
Departament d'Anatomia i Embriologia Humana 
Facultat de Medicina (HCP) 
Universitat de Barcelona 
ICQ 14146798 

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