[Histonet] Re: Dystrophin antibody

[Andi Kappeler]

Hi Cheryl
we use clone 13H6 (Novocastra, NCL-DYSA) at 1:50 after HIER in 100 mM Tris -
5% Urea, pH 9.5. Other high pH retrieval buffers may work as well. Hope this
helps.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "Cheryl Crowder" <ccrowder <@t> mail.vetmed.lsu.edu>
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Sent: Wednesday, April 14, 2004 5:58 PM
Subject: [Histonet] Dystrophin antibody


> Hi - I have a researcher who would like to do Dystrophin IHC on
> formalin-fixed, paraffin embedded tissue.  Does anyone know of an antibody
> which will work.  Thank you, Cheryl
>
>
> Cheryl Crowder, BA, HTL(ASCP)
> Chief Technologist
> Anatomic Pathology
> Department of Pathobiological Sciences
> School of Veterinary Medicine
> Louisiana State University
> Skip Bertman Drive
> Baton Rouge, LA  70803
>
> 225-578-9734
> FAX:  225-578-9720
>
>
>
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