[Histonet] Safranine o staining and Problem in tissues

[balajimr <@t> drreddys.com]

Can anyone give me the detailed procedure for safranine o staining for the 
joints (ankle. tarsal and metatarsal joints in rats). I would like to know 
a good  decalcifying solution for the same and what is the duration of 
bone to be kept in the decalcifier. 


Secondly, of late I am facing  peculiar problem now with my liver, kidney 
and brain sections of late. These sections are cracking after the staining 
(Mayer's H& E). It is something like parched earth appearance. This I am 
noticing when the tissue s are coming to last xylenes. I donot see the 
cracking before this step. This doesn't happen in all the slides. Could 
you please suggest some solution for this. We are using chloroform for 
clearing and histoplast from  Shandon for infiltration. I am unable to 
locate where exactly is the problem. I have tried changing the makes of 
chloroform and xylenes used with out much help. 



Dr. Balaji  (M. V. Sc. Path)
Dept. Pre clinical safety evaluation,
Discovery research, 
Dr. Reddys Laboratories ltd. Bollaram Road, 
Miyapur, Hyderabad, 500 049
 Andhra Pradseh, INDIA

Tel: 23045439 - Ext.432.