[Histonet] Vibratome sections and immunohistochemistry

[Gayle Callis]

People do this all the time, and this is found in literature.  A PUBMED
search will help you glean a lot of knowledge, at least methods. Access
this publication: Zhuo L et al, Live astrocytes visualized by green
fluorescent protein in transgenic mice, Developmental Biology 187:36-42,
1997.  

They did 300 um vibrotome section on brain, far thicker than you want to
use.  Even though this is for GFP and fluorescent work, their methods and
materials were excellent. 

 

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)