[Histonet] Melanin Bleach & IHC

[Bonnie Whitaker]

There are also other chromogens available for HRP that aren't "DAB brown".
I still like AEC, but Vector has several others now.

Bonnie Whitaker
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
DDittus787 <@t> aol.com
Sent: Wednesday, April 07, 2004 4:24 PM
To: LBlack <@t> carilion.com; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Melanin Bleach & IHC

lisa;
instead of using the bleaching, or worrying that the dab looks like melanin,
protect your tissue and the results by using alk phos fast red detection. it
will stain red, is permanent and can be used on almost every antibody with
the exception of ER/PR and if I recall correctly(ventana will tell me if its
not) Alk Phos fast Red is a bit more sensitive for detection than dab, the
pathologist will adjust i assure you and morphology is saved.          dana

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