[Histonet] Sponge problems in processing

Laurie Colbert laurie.colbert <@t> huntingtonhospital.com
Wed Apr 7 11:01:17 CDT 2004

I just attended a training session for our new VIP at Sakura and was told that the function of the vacuum is in fact to only "suck away" the air bubbles that cling to the tissue.  This allows the solutions to come in contact with all surfaces of the tissue.  I learned something new!!

Laurie Colbert
Huntington Hospital
Pasadena, CA

-----Original Message-----
From: Kemlo Rogerson [mailto:kemlo <@t> tiscali.co.uk]
Sent: Wednesday, April 07, 2004 8:48 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Sponge problems in processing

I thought vacuum processing only 'sucked away' air. I mean if you reduce
the air pressure above the fluids then that reduces pressure within the
tissue that was preventing air escaping (as it has a positive pressure).
I suppose if you reduce the Saturated Vapour Pressure of a solvent then
that would cause more solvent to vapourise but wouldn't that be from the
surface of the solvent rather than within the tissue? 

Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS
Tel: 0208 970 8414
Mob: 07830 196072 
Mobile E-Mail kemlorogerson <@t> 3mail.com                     
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall
Terry Dr,Consultant Histopathologist
Sent: 06 April 2004 16:21
To: Steven E. Slap; Gayle Callis; Gudrun Lang;
Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Sponge problems in processing

OK. 200 ml. of liquid retained, with 100 blocks.
However, with a vacuum processor, is that not all "sucked away"?

BTW, should it sound otherwise, I hate the things (but am stuck with

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Steven E. Slap [mailto:siksik03 <@t> comcast.net]
Sent: 06 April 2004 16:14
To: Gayle Callis; Gudrun Lang; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Sponge problems in processing

Hi HistoNetters

I agree with Gayle, and the other posters who pointed out that 
sponges carry over 1ml of fluid per sponge (so, with 100 cassettes, 
200 sponges, that's a lot of carryover).

I have had a lot of success with the biopsy cassettes which Gayle 
refers to, both in microwaves and in conventional tissue processors. 
You can request samples from Lab Storage Systems in St. Louis by 
phone at (800) 345-4167 or by e-mailing Rita Lovshe at 
rcl <@t> labstore.com.

best regards,
Steven Slap

At 10:20 AM -0700 4/1/04, Gayle Callis wrote:
>To: "Gudrun Lang" <gudrun.lang <@t> aon.at>,
Histonet <@t> lists.utsouthwestern.edu
>From: Gayle Callis <gcallis <@t> montana.edu>
>Subject: [Histonet] Sponge problems in processing
>We no longer use sponges, prefer to place tissues in tissue embedding
>(Fisher) which look like tea bags or the little nylon bags (not as easy
>handle, but thinner than sponges) and a tidge stiffer than tea bags.
>Sponges can cause artifacts in your tissues, looking like triangular
>in section.  This was published by Freida Carson in Journal of
>Histotechnology, 1980's.  Using tea bags may not be as fast during
>embedding, but speed there is a trade off for having to reprocess
>tissue samples, ho hum tedious and time consuming while patient waits. 
>You analysis of problem sound correct with a poor exchange of solvents
>through sponges. If you pack cassettes super tight in basket/cassette
>holder inside processor you can impede solvent flow or if sponges are
>crammed too tight agaist tissue then lid smashed down on tissue/sponge
>sandwich - this is like having too thick a tissue in a cassette. 
>There are some clever biopsy cassettes with a folding, fine mesh
>fitting inside a cassette.  These may be worth a try to avoid sponges.
>think they are available through Fisher or some other company who could
>provide free samples.  It will be interesting to see peoples
>on using these. 
>There was Histonet discussion on these sponges way back in time - it
>pay to do a search for those messages in Histonet Archives.

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