[Histonet] microwave antigen retrieval

Steven E. Slap siksik03 <@t> comcast.net
Tue Apr 6 10:19:33 CDT 2004

Hi HistoNetters

Successful antigen retrieval depends on accurate 
control of three factors:  time, temperature and 
pH.  Any instrument which will produce this 
accuracy in a reproducible way will be effective. 
A microwave has been shown to work well over a 
wide range of antigens, where other methods have 
required more aggressive treatment.  In some 
cases, pressure is needed to allow for 
temperatures above 98°C.  I can get most antigens 
to "work" with pH 6.0 citrate buffer at 110°C in 
a 3-5 minute microwave method using the 
Hacker/Milestone microwave.

best regards,
Steven Slap
Microwave Consultant

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