[Histonet] Help with frozen section staining
Barry R Rittman
Barry.R.Rittman <@t> uth.tmc.edu
Fri Apr 2 08:07:19 CST 2004
Pierre et al
Hi to y'all in Canada, I have a relative in Brampton, Ontario and really enjoy my visits there.
A point re OCT compound. It contains several salts including I believe some calcium. It is important therefore for some procedures such as when using certain lectins to thoroughly remove the OCT from the section to prevent interference with staining and with other procedures. This is easily taken care of with buffer/or alcohol etc.
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Greg Dobbin
Sent: Friday, April 02, 2004 3:02 AM
To: piernoel <@t> health.nb.ca
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Help with frozen section staining
Hi Pierre-André (neighbor),
I don't see the OCT being the most likely culprit, unless MAYBE if
you were perfusing with it. The OCT should only be surrounding
your tissue sections (not entering into them). I would be more
inclined to look at:
a) the fixative (try brand new batch, if the fixative is faulty, your
sections will not tolerate the non-isotonic and perhaps acid or basic
pH of your staining solutions). And then
b) the mounting medium (for clarity) and the coverslips for defects.
(presumably you've ruled out the microscope as the problem?) Good luck. Greg
Date sent: Fri, 02 Apr 2004 08:28:01 -0400
From: piernoel <@t> health.nb.ca (Pierre Noel)
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Subject: [Histonet] Help with frozen section staining
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> Hi all,
> We just recently notice a major problem with our frozen section
> staining. The cellular morphology is poor, haziness and no cellular
> definition. We have tried different staining techniques with different
> fixative but no luck. Could it be our OCT? We desperately need some
> input on the subject. Thank you!
> Pierre-André Noel
> Histology Supervisor
> Bathurst Regional Hospital
> NB, Canada
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