[Histonet] non-speficic binding of secundary biotinilated antibodies

[Bruijntjes, J.P.]

Hi all

In the past I used a rabbit anti mouse biotinilated secondary antibody
with my lymphocyte-monoclonals on fresh frozen spleen and/or thymus
slides of the rat. But I always had to add some ul's normal rat serum to
avoid non-specific binding of this reagent to lymphocytes. 
Is anyone of you aware of a good secondary step in which adding of
normal rat serum is not necessary anymore? 

Joost Bruijntjes
TNO Nutrition and Food Research
PO Box 360
3700 AJ
Zeist
The Netherlands


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