[Histonet] Kappa, Lambda staining???

[Sebree Linda A.]

Hello Jesus,
 
You don't say whether you are using polyclonal or monoclonal antibodies.  It makes a difference.  We use monoclonal kappa and lambda from Biocare on our Benchmark with nice clean results.  Let me know if you'd like the protocols.
 
Linda Sebree, HT(ASCP)
University of Wisconsin Hospital and Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
la.sebree <@t> hosp.wisc.edu
	-----Original Message----- 
	From: Jesus Ellin [mailto:Ellin13 <@t> adelphia.net] 
	Sent: Tue 9/30/2003 7:00 PM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] Kappa, Lambda staining???
	
	
	Hello everyone ,, Need some help here, currently our lab is trying to create a IHC protocol for kappa and lambda on the Venntana benchmark,, we are having some real problems with the background staining in our control and patients,, we are using a blocker and or hydrogen peroxide, to try and minimize the peroxidase but we are still getting alot of background,  if any one out there that has a benchmark and has a protocol for these stains some help would be appreciated,, forgot to mention that we are using Tonsil and Bone Marrow for control,
	 
	One other thing how many out there are using the Tamtron System,, we are currently in the building phase and would like to ask some people out there questions concerning proccess, codes and end user training
	 
	 
	Jesus Ellin HTL ASCP    
	Yuma Regional Medical Center
	jellin <@t> yumaregional.org