[Histonet] Re:Excessively Wrinkled sections

[Albert Grobe]

Gillian,
Since your samples have been through the 60C paraffin, raising the waterbath temperature a few degrees should not affect you epitopes.  Ideally, the waterbath should be a couple degrees below the melting temp of the wax but some fine adjustment is sometimes necessary.  I have had the same problem in the past, and raising the temp has helped.
Albert

Albert C. Grobe, MS
Tissue Engineering Lab
International Heart Institute
Missoula, MT 59802
Office:  (406) 329-5634
E-Mail:  GrobeA <@t> saintpatrick.org