[Histonet] Isopropyl alcohol and paraffin wax

Jenny Oblander Jenny-Oblander <@t> omrf.ouhsc.edu
Thu Sep 25 14:44:50 CDT 2003


When microwaving is used in histoprocessing, these procedures are not only
accelerated, but fundamentally changed:

Completion of fixation is achieved prior to histoprocessing in the microwave
oven. 
Dehydration is achieved in one step, instead of the 2-6 steps used in
conventional procedures. The use of a graded series of alcohols is not
necessary in the microwave method. 
Isopropanol can be substituted for xylene as a clearing agent, and one bath
is sufficient. 
Higher temperatures are required for the impregnation of the paraffin wax. 
Therefore, microwave histoprocessing becomes a three-step process:

One dehydration step 
One clearing step 
One paraffin wax step (at two temperatures) 

This is from Microwave technique- Energy Beam Sciences
-----Original Message-----
From: Smith, Allen [mailto:asmith <@t> mail.barry.edu]
Sent: Thursday, September 25, 2003 1:18 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Isopropyl alcohol and paraffin wax


Miscible means mutually soluble in ALL proportions.
Isopropyl alcohol and paraffin was are definitely not miscible.
However, they are mutually soluble to a limited degree.
I just did a few crude solubility estimates by eyeball in my lab:
I got about half a gram of "Paraplast" to dissolve in 100 ml of gently
boiling isopropyl alcohol.
I was able to get 30 ml of isopropyl alcohol to dissolve in 100 ml of molten
"Paraplast".
Both solutions become cloudy suspensions on cooling.
I have not tried transferring tissue directly from isopropyl alcohol to
molten paraffin, but it seems that the experiment would be worth trying.

Allen A. Smith, Ph.D.
Professor of Anatomy
School of Graduate Medical Sciences 
Barry University 
Miami Shores, FL
           
                       
                
The information transmitted is intended only for the person or entity to
which it is addressed and may contain confidential, and/or privileged
material. No confidentiality or privilege is waived or lost by any errant
transmission. If you receive this message in error, please immediately
delete it and all copies of it from your system and notify the sender.
E-mail transmission cannot be guaranteed to be secure or error-free as
information could be intercepted, corrupted, lost, destroyed, arrive late or
incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu) 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




More information about the Histonet mailing list