[Histonet] caspase-3

[Luz Cardenas]

Hi all,
 I would also like to have the protocol...I am just starting to try in 4% Paraformaldehyde fixed mouse brain sections...
Also I read is difficult to get the staining.  some kind of special conditions?
Thanks!


Luz Cárdenas, MD
Post-doctoral Research Trainee
Department of Anatomy and Neurobiology
University of Tennessee Health Science Center
855 Monroe Avenue, Room 309/302
Memphis, TN 38163
Lab: (901) 448-6002 / 7318
Fax: (901) 448-7193 (Dept)



> Hi everyone!
> 
> Is anyone using cleaved caspase-3 with a protocol other than 
> overnight at 
> 4 degrees?  I'm having problems with my staining and I don't have 
> weeks to 
> get it sorted out, so I am looking for a room temp or heated 
> protocol to 
> try.
> 
> Thanks in advance
> 
> Noelle Linke, BS, HTL(ASCP)
> Allergan, Inc
> Irvine, CA 92612
> 
> 
>