[Histonet] MiTF

[marjorie lehman]

Hi,
Has anyone stained for microthalmia transcription factor in frozen skin?
I have tried Abs from 2 vendors, diluted down to 1:10, changed diluting fluids,
used a vector kit, made my own solutions, incubated 1 hr, 2hrs and overnight
and I'm still getting nothing.  I'm using pigmented human skin which is the
suggested control. Any help would be appreciated.
Thanks,
Marge