[Histonet] Re: Brain Tissue in Buffer

[Frouwke Kuijpers]

My experience is that when fixed brain stays long in PBS, it becomes very
soft. Than I don't use these brains anymore.
Reason? I don't know. Somebody else maybe?

Frouwke Kuijpers

----- Original Message ----- 
From: <Nancy.Walker <@t> sanofi-synthelabo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, September 23, 2003 1:36 PM
Subject: [Histonet] Re: Brain Tissue in Buffer


>
> Hi,
> I've been told that if you fix in PFA then store in PBS for long periods
> the PBS "unfixes" the tissue. So I would suggest redoing your fixation
> process.
>
> Good luck!
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>