[Histonet] Re.Isopropanol in processing

[John Kiernan]

Dear All including John D.,

Isopropanol is not miscible with melted
paraffin wax. If you don't believe the 
usual published references you can easily 
check for yourself. Add a drop of melted 
wax to a small beaker containing warm 
isopropyl alcohol. It won't dissolve,
even if you heat to higher than the
melting point of the wax.
 
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
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John Difford wrote:
> 
>  Dear All
> 
> Despite some statements to the contrary that have recently been posted on
> Histonet, I have to inform you that isopropanol is miscible with melted
> paraffin wax and this is why one can use if for processing without the use
> of a clearing agent.
> 
> John Difford
> Histopathology
> Royal Free Hospital
> London
> England, UK
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