[Histonet] Re: Reply- isopropanol as dehydrator in microwave method
John Kiernan
jkiernan <@t> uwo.ca
Tue Sep 23 10:43:44 CDT 2003
Someone wrote:
> It is actually better to use isopropanol
> as the final step between the alcohol
> and paraffin, since it has a lower
> boiling point, it evaporates out faster
> than ethanol, ...
Merck Index says the boiling points
are:
Ethanol 78.5
Isopropanol 82.5
There has to be another reason why you
can get away without using a clearing
agent!
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan <@t> uwo.ca
http://publish.uwo.ca/~jkiernan/
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