[Histonet] SAB for amyloid
Tony Henwood
AnthonyH <@t> chw.edu.au
Thu Sep 18 18:11:05 CDT 2003
The technique is as follows:
SULPHATED ALCIAN BLUE FOR AMYLOID
PRINCIPLE:
Amyloid has an increased avidity for alcian blue in the presence of added
salt. By fixing with an akali solution, SAB can be followed by various
trichromic counterstains.
FIXATION: 10% buffered formalin
MICROTOMY: 7um paraffin sections
SOLUTIONS:
1. Acetic/Alcohol Rinse:
95% ethanol 45ml
Distilled water 45ml
Glacial Acetic Acid 10ml
2. Stock Solutions:
a) 1% Alcian Blue (CI 74240) in 95% ethanol.
b) 1% Sodium Sulphate Hydrate in distilled water.
3. SAB Working Solution - prepare fresh:
Stock solution (a) 18ml
Stock solution (b) 18ml
Glacial Acetic Acid 4ml
Mix, stand 30 minutes before use.
4. 80% ethanol saturated with borax.
5. Van Gieson counterstain.
METHOD:
1. Hydrate section and wash for 10 minutes.
2. Rinse in acetic/alcohol, 2 minutes.
3. Place in SAB, 2 hours.
4. Rinse in acetic/alcohol, 2 minutes.
5. Wash in water.
6. Alkalinise in borax/alcohol, 30 minutes.
7. Wash in water.
8. Counterstain in Van Giesons.
9. Blot dry, dehydrate, clear and mount.
RESULTS:
Amyloid blue-green
Collagen red
Muscle, cytoplasm yellow
Mast cell granules and some colloids are also blue-green.
REFERENCE:
Lendrum, A.C., Slidders, W., Fraser, D.S., (1972). "Renal Hyalin: A Study
of Amyloidosis and Diabetic Fibrinous Vasculosis with new Staining
Methods". J. Clin. Path. 25, 373-396.
This is from an old manual. Sorry that the Hazardous warning phrases are
missing.
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----
From: rfail <@t> toolkitmail.com [mailto:rfail <@t> toolkitmail.com]
Sent: Friday, 19 September 2003 6:13
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] SAB for amyloid
Does anyone know of an amyloid stain with the abbreviation SAB?
Rena Fail
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