[Histonet] Purpose of Oven Prior to Staining?

[Tony Henwood]

Jordan,

Reasons for drying slides above the melting point of wax:
1.	Remove excess water
2.	Allow sections to attach to slides (otherwise they will lift off in
the dewaxing xylene).
3.	Aid in final spreading out of sections so that there are fewer folds
(should I say NO folds!!)
4.	Remove some of the excess wax (ie it flows off the slide). Remember
this requires regular cleaning of the oven to remove excess wax.
5.	Can't think of any other reasons

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html




-----Original Message-----
From: Jordan Brod [mailto:jbrod <@t> tvmdl.tamu.edu]
Sent: Friday, 19 September 2003 0:41
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Purpose of Oven Prior to Staining?


Good Morning All:

My lab is in a huge discussion about the reason why we put our slides in an
oven before staining.  I told them that the reason was to dry all of the
water off of them, because xylene and water do not mix.  This statement is
also backed up in literature that I have read.  They still do not believe
me, because they think the purpose of the oven is to deparaffinize the
slides.

Can anyone help me out, so I can put this discussion to rest?  I will print
off all of your comments for my technicians to read.  Is what I told them
correct or not?

Thanks.


 

********************************************************************
Jordan W. Brod
Diagnostic Lab Supervisor - Pathology
Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
1 Sippel Road
TAMU 4471
College Station, Texas 77843
(979) 845-3414
(979) 845-1794 fax
jbrod <@t> tvmdl.tamu.edu


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