[Histonet] Purpose of Oven Prior to Staining?

[Bartlett, Jeanine]

I agree about the removal of water.  It also can aid in helping sections
adhere to slides, but if you are using "plus" slides that aspect is probably
irrelevant.

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333


-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Sent: Thursday, September 18, 2003 11:37 AM
To: Jordan Brod
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Purpose of Oven Prior to Staining?


Hi Jordan:

    I'm with you on this one. If the oven will deparafinize sections it 
should be possible to stain sections with an aqueous stain after 
"deparafinization" in the oven. Have them try this while you remove wax 
the normal way. Compare results. Maybe then they will believe you 
(unelss they are very stubborn).

Geoff

Jordan Brod wrote:

>Good Morning All:
>
>My lab is in a huge discussion about the reason why we put our slides 
>in an oven before staining.  I told them that the reason was to dry all 
>of the water off of them, because xylene and water do not mix.  This 
>statement is also backed up in literature that I have read.  They still 
>do not believe me, because they think the purpose of the oven is to 
>deparaffinize the slides.
>
>Can anyone help me out, so I can put this discussion to rest?  I will 
>print off all of your comments for my technicians to read.  Is what I 
>told them correct or not?
>
>Thanks.
>
>
> 
>
>********************************************************************
>Jordan W. Brod
>Diagnostic Lab Supervisor - Pathology
>Texas Veterinary Medical Diagnostic Laboratory (TVMDL)
>1 Sippel Road
>TAMU 4471
>College Station, Texas 77843
>(979) 845-3414
>(979) 845-1794 fax
>jbrod <@t> tvmdl.tamu.edu
>
>
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>  
>

-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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