[Histonet] Reply on blood clot sections

[Gayle Callis]

We let 10 mls blood clot (let it sit for a time, 1 hour was minimal, longer
gave a firmer clot) and using razor blade, cut it into sections, froze the
clot "slabs" using the heat extractor and did frozen sections fixed the
sections with NBF and did oil red o staining for fat embolism in blunt
truama/broken bones from accident patients.  It was not the easiest thing
to cut and experience with large blood clots inside distended blood vessels
or areas of hemorrhage found within FFPE embedded weren't fun to section
after routine tissue processing. Generally, the tissue block had to be
soaked in ice water for optimal sectioning.   

At 12:42 PM 9/17/2003 -0400, you wrote:
>Hi Histonetters,
>Does anybody has an experience in preparation of clot sections?
>
>Thanks
>Andrew Fedanov Ph.D.
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)