[Histonet] BrdU and EM?

[Mezey Szilvia]

 Animal tissue (domestic chicks), transcardially perfused.

 Szilvi

Subject:        	RE: [Histonet] BrdU and EM?
Date sent:      	Wed, 17 Sep 2003 08:03:03 -0500
From:           	"Charles W. Scouten, Ph.D." <cwscouten <@t> myneurolab.com>
To:             	"Mezey Szilvia" <mezey <@t> ana.sote.hu>

> Animal tissue (perfused?) or human tissue?
> 
> Charles W.  Scouten, Ph.D. 
> myNeuroLab.com 
> 5918 Evergreen Blvd. 
> St. Louis, MO 63134 
> Ph: 314 522 0300  
> FAX  314 522 0377 
> cwscouten <@t> myneurolab.com 
> www.myneurolab.com 
> 
> 
> -----Original Message-----
> From: Mezey Szilvia [mailto:mezey <@t> ana.sote.hu] 
> Sent: Wednesday, September 17, 2003 6:54 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] BrdU and EM?
> 
>  Hello All,
> 
>  I'm trying to do BrdU staining (in paraformaldehyde fixed, free-
> floating sections) and preserve the ultrastructure of the tissue for 
> EM at the same time. HCl works fine for denaturing DNA for BrdU-
> ICC but doesn't leave much of the tissue for EM. DNase would be 
> more EM-friendly but doesn't penetrate the tissue enough.
>  
>  Does anybody know a method that could provide a fair enough 
> compromise between BrdU and EM? Maybe by increasing the 
> penetration of DNase?
> 
>  Best regards to you all,
> 
>  Szilvi
> 
> 
> Szilvia Mezey
> PhD student
> Semmelweis University
> Dept. of Anatomy, Histology and Embryology
> Tuzolto u. 58. Budapest, 1094, Hungary
> T.: +36-12156920/3687
> F.: +36-12155158
> E-mail: mezey <@t> ana.sote.hu
> 
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Szilvia Mezey
PhD student
Semmelweis University
Dept. of Anatomy, Histology and Embryology
Tuzolto u. 58. Budapest, 1094, Hungary
T.: +36-12156920/3687
F.: +36-12155158
E-mail: mezey <@t> ana.sote.hu