[Histonet] Bouin's fixation/removal of picric acid

[Sarah Jones]

I use it on wet tissue, it does not appear that the lithium needs to be
removed.  Maybe I'm not understanding your question.   Sarah
Ref. Theory and Practice of Histotechnology, Sheehan, Hrapchak, page
47.    


>>> John Kiernan <jkiernan <@t> uwo.ca> 09/14/03 11:54PM >>>
Sarah, do you use the saturated lithium 
carbonate in 70% ethanol on blocks of
tissue or on hydrated paraffin sections
that are yellow?  

If it's for blocks, how do you remove
lithium carbonate from the decolorized
specimens?  
              John Kiernan
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca 
   http://publish.uwo.ca/~jkiernan/ 
___________
Sarah Jones wrote:
> 
> I've always used saturated lithium carbonate in 70% ethanol to
remove
> the picric acid.  I believe it would be faster and more complete
than
> 70% alone.
> 
> Sarah Jones HT(ASCP)
> Dept. of Vet. Anatomy & Public Health
> Histology Lab
> Texas A&M University
> College Station, TX 77843-4458
> phone: 979-845-3177
> fax:  979-458-3499
> 
> >>> "peptolab" <peptolab <@t> hamptons.com> 09/13/03 08:32AM >>>
> A 3-5 mm testicular biopsy should be fixed in one to two hours. Be
sure
> to
> rinse out as much yellow picric acid as possible (you can use
running
> water
> or 70% alcohol ). The basophilia of chromatin in the stored block
will
> deteriorate after some time if there is picric acid  residue.
> 
> Jeff Silverman HT HTL QIHC (ASCP)
> Southside Hospital
> Bay Shore NY
>