[Histonet] validating oil red O

[Paul Howard Lockwood]

Dear Jeremy,
     I would suggest using frozen sections, and mounting them on slides coated with poly-l lysine. Also, do not expose the slides to heat above room tempeture.
     I hope this is of some help to you.
     Sincerely,
     Paul Lockwood
9/11/03 6:53:05 PM, Jeremy Browne <j.browne <@t> auckland.ac.nz> wrote:

>
>
>
>   We need to set up a range of fat concentrations on slides, stain and then
>   check to see that there is a linear response relationship between fat
>   concentration and staining intensity but because fat melts and slides off
>   histoworld has solved this problem - perhaps with an intermediatere is
>   substance to which the fat adheres?
>   Thanks
>   Jeremy Browne
>   Liggins Institute
>   NZ (09) 373 7599 extn 86444
>   j.browne <@t> auckland.ac.nz