[Histonet] nuclear counterstain
Atoska S. Gentry
gentras <@t> vetmed.auburn.edu
Fri Sep 12 10:15:22 CDT 2003
Hello again, guess I need to rephrase my initial inquiry on the
counterstain to use with the Vectastain ABC-HRP kit. But, first special
thanks to Dr. John Kiernan. He always replies with such informative and
thorough remarks. Please pardon my partial entry of facts. This inquiry is
on behalf of a couple of colleagues of mine. And yes their work was with
monolayers of cultured bone marrow cells. However, the HRP was detected
with the Vector VIP substrate ( for which Vector Labs does not reveal
the contents) and yielded neurons that are purple in color. Therefore, we
need something like acridine orange which stains DNA. Has anyone had any
experience/success using acridine orange or something similar with this
system that yields the same results? Thanks, Atoska
Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL 36849
Phone# (334)844-5579 Fax# (334)844-5850
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