[Histonet] nuclear counterstain

[Atoska S. Gentry]

Hello again, guess I need to rephrase my initial inquiry on the 
counterstain to use with the Vectastain ABC-HRP kit. But, first special 
thanks to Dr. John Kiernan. He always replies with such informative and 
thorough remarks. Please pardon my partial entry of facts. This inquiry is 
on behalf of a couple of colleagues of mine. And yes their work was with 
monolayers of cultured bone marrow cells. However, the HRP was detected 
with the Vector VIP substrate       ( for which Vector Labs does not reveal 
the contents) and yielded neurons that are purple in color. Therefore, we 
need something like acridine orange which stains DNA. Has anyone had any 
experience/success using acridine orange or something similar with this 
system that yields the same results? Thanks, Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850