[Histonet] TESTICULAR BIOSY

[John Kiernan]

It doesn't make sense to transfer a specimen
from Bouin to a neutral formaldehyde solution.
The usual way to use Bouin is to fix overnight
and then transfer the specimens to 70% or 95%
alcohol - several changes, to extract as much
yellow colour (picric acid) as is reasonably
possible. Then complete the dehydration, clear,
infiltrate with wax and embed in the usual way.

The tissue will be properly fixed after only a few
hours in Bouin - a major difference from neutral
formaldehyde. The nuclear morphology will be
clearer than you'll ever see after formaldehyde
alone, and connective tissue stains (trichromes 
etc) will give really bright colours. 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
____________________________
> Travis Troyer wrote:
> 
> Our lab is receiving a testicular biopsy that will be
> received in Bouin's Solution.  We routinely place
> specimans in 10% NBF after grossing.  So, my question
> is how long should the biopsy stay in the Bouin's
> solution for proper fixation before placing into 10%
> NBF?
> Thanks!