[Histonet] de-staining immunos

[Tony Henwood]

Amy,

Diff-quik slides can be destained. Place in 1%acetic acid in ethanol. This
will remove most of the dyes. 

As for IP stained slides, unless an ethanol soluble substrate is used (eg
AEC), it may be very difficult to remove the DAB substrate. You could try 5
min treatment with KMnO4 followed by Oxalic acid, though I can't be certain
this will work.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Amy Self [mailto:ASelf <@t> gmhsc.com]
Sent: Wednesday, 10 September 2003 20:02
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] de-staining immunos


	
		
	Does anyone out in histoland know if Immuno stained slides and/or
air-dried Diff-Quik stained slides can be de-stained? If so could you please
share with me this procedure.  I know that H&E's can be de-stained but I
wasn't sure about immunos and diff-quiks.  Thanks for the help....


	Amy Self
	Georgetown Memorial Hospital
	Georgetown, SC
	(phone) 843-527-7179
	(fax) 843-520-7882


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