[Histonet] cleaning microtomes

[Fred Underwood]

I find that a dry gauze does the job.   I have tried paragard, however,
and it seems that the paraffin that accumulates on the paragard cleaned
surface seems to be sticky and more difficult to clean.  Has anyone else
had this experience, or am I on an island here?

Fred

>>> "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov> 09/09/03
11:31AM >>>
I use paragard which is just oil of wintergreen, followed by ETOH. Do
not
use xylene because of health concerns. I would be interested in what
Gayle
Callis has to say, I believe she took a course at NSH previously and
the
instructor recommended not using any clearing agent. Would be
interesting to
have a manufacturers recommendation.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Jmrwalker <@t> aol.com [mailto:Jmrwalker <@t> aol.com] 
Sent: Tuesday, September 09, 2003 8:49 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] cleaning microtomes


I have a question that may seem silly.  After you are finished cutting
on 
your microtome, what do you use to clean it?  Our lab in the past
always
used 
xylene or alcohol to clean it.  A few new people have been hired in the
lab
and 
now Paragard and baby oil have been used.  I'm just curious what the
majority 
of people in histoland are using.  Thanks!

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