[Histonet] kappa and lambda staining

[Richard Cartun]

We published our routine method in, "Immunohistochemical detection of
immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas
using formalin fixed, paraffin-embedded tissues and a heat-induced
epitope retrieval technique" that appeared in Applied
Immunohistochemistry & Molecular Morphology 10(3):258-262, 2002. 
However, I can tell you that we use different retrieval methods and
different primary antibody dilutions when the initial results are
suboptimal.

Richard Cartun

>>> "Martha Ward" <mward <@t> wfubmc.edu> 09/10/03 11:50AM >>>
I was wondering if anyone has any tips on kappa and lambda staining on
bone marrow and bone plug sections?  My pathologists say we are getting
inconsistant staining on these specimens.  Our histology lab puts the
bone marrows in B5 solution for 2 hours and then into formalin for
processing.  The bone plugs go into B5 for 2 hours and then into
DeltaCal (from Delta Products Group).  We are currently using a NexEs
for our staining and a BioCare monoclonal Kappa and Lambda antibody,
using antigen retrieval.  I also have recently installed a Dako
Autostainer.  I would appreciate any feedback for either systems. 
Thanks in advance.

Martha Ward, MT (ASCP) QIHC
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center

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