[Histonet] sudan black B

[Jose Luis Palazon Fernandez]

Dear List members. 

I posted this question some weeks before but had no answer. As part of my thesis I have to study lipids. I stained some slides with sudan black B. I used 4 slides per specimen and I did (delipidized, sudan black B, Bromide-sudan black, and bromide-acetone-sudan black). I have noticed that the slides were clearly black after staining, the bromide treated the most black, but after 1-2 weeks, they tured green in color and difusing in the borders of the tissue. I used an aqueous mounting media. Have any of you working with lipids had a similar situation? Is the color of sudan black B stained slides not permanent? must the slides be read inmediately? do this change in color alter the results if I read some recently stained slides and other stained some weeks ago?. Is it possible (adequate) to put the coverlips out and re-stain the slides? I would appreciate some advice from you. 

Thanks in advance

José Luis