[Histonet] CD1a

[Galbraith, Joe]

Steve:

We use CD1A from Immunotech on a Dako Autostainer (15-15 program), dilution
neat (ouch) with full power (600W) microwave hier for 8 minutes with a 15
min cool down then into Dako wash solution for 5 minutes prior to loading on
the instrument.  We get good results without background.

Good luck,

Joe Galbraith

-----Original Message-----
From: Steve Machin UK [mailto:stevemachinuk <@t> yahoo.co.uk]
Sent: Monday, September 08, 2003 11:04 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] CD1a


We are having trouble with our CD1a from DAKO.
We use microwave for 20 mins and get background and very weak
staining.
What method do others use?

Best Wishes

Steve Machin UK

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