[Histonet] Cryoprotection

[John Kiernan]

The solution you describe is not a cryoprotectant.
It is an anti-freeze. 

A cryoprotectant is used before freezing and cutting 
sections, to reduce the sizes of the ice crystals in 
the tissue. 30% sucrose in water is a commonly used
cryoprotectant for blocks of fixed tissue.
-- 
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
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Frouwke Kuijpers wrote:
> 
> Has anybody has experience with this Cryoprotect-solution :
> 0,05 Sodium phosphate buffer
> 30% ethylen glycol
> 20% glycerol
> for storing 40 micron sections at - 20 C
> 
> Has somebody experiences with this?
> We don't want to put them on glasses because we do FFM.
> 
> F.J.Kuijpers-Kwant
> Dept. Cellular Animal Physiology
> University of Nijmegen
> Toernooiveld 1
> 6525 ED Nijmegen
> frouwke <@t> sci.kun.nl
> 
> _______________________________________________