[Histonet] animal incubation time??

[Sarah Jones]

Hi Dave,
  I think we need more info before we can answer this question.  Are you processing without an automated tissue processor?  I assume you are talking about paraffin infiltration when you refer to incubating time in an oven.  Is that correct?  We would need to know how big the tissue is you are infiltrating to know if the times are correct.  Can you tell us what the species and dimensions are?  And I have no idea what you mean by "Also if paraffin is on the slides won't the xylene/histoclear remove that?"  What is that refering to exactly?  
  One thing I can tell you is infiltration is not one of the steps I like to shorten.  Are you using several stations of paraffin during this 2 hour period?    Thanks, Sarah


Sarah Jones HT(ASCP)
Dept. of Vet. Anatomy & Public Health
Histology Lab
Texas A&M University
College Station, TX 77843-4458
phone: 979-845-3177
fax:  979-458-3499


>>> "Dave McClister" <davenhv <@t> hotmail.com> 09/05/03 09:25AM >>>
Thanks for all the info on the animal processing.  The incubation time in 
the oven has been 2 hours for the animal hearts.  I have always just put 
them in the oven (62 C) for 25 min for surgicals, 40 min for bone marrow, 
and 60 min for autopsy.  Can this two hour incubation time be cut down as 
long as no water is visible? Also if paraffin is on the slides won't the 
xylene/histoclear remove that?


Dave McClister
davenhv <@t> hotmail.com 

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