[Histonet] Sirius red

[Patsy Ruegg]

Andi,
When I was at the U I got Sirius Red thus:
It is made by Chroma Gsellschaft Schmid
GmBH + Co. D-7316 Konegen/N West Germany
Distributed in the US by Roboz Surgical Instrument Co. Inc.  100 Connecticut
Avenue N.W. Washington, D.C. 20036
Haven't bought it in years.  I did just recently used a Picro-Sirius Red 
cat.# s2365 from Polyscientific, not sure of the C.I. # but it worked just 
like the stuff I had used before from Germany.  Did you try the one's from 
the other places or did you just think that it would not work per your 
investigator?  Perhaps they did not mix it with saturated picric acid, you 
need that for it to work properly.  I do an alcian blue counter stain before 
the picro-sirius stain and it is beautiful.
Patsy




Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg <@t> msn.com
wk email pruegg <@t> colobio.com

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----Original Message Follows----
From: Andrea Grantham <algranth <@t> u.arizona.edu>
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sirius red
Date: Thu, 30 Oct 2003 15:08:08 -0700

I have a quick question about Sirius red since it is a recent topic of 
discussion on histonet.
I was asked to do the stain and given a protocol calling for Sirius red F3B 
(CI 35782). I have Sirius red CI 35780. They said that they have tried the 
stain using CI 35780 and it didn't work but they cannot find the other 
Sirius red with a CI# 35782 in any of the catalogs. We have tried Sigma, 
Polysciences, PolyScientific, Newcomer, and some others...
Any suggestions where I can find it?
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
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