[Histonet] collagen I staining

[Rita Angel]

Hello Histonetters,

Someone in our lab has been trying to stain for collagen I antibody in 
rabbit tissue. She's tried the antibody from several different companies 
and has used different antigen retrievals, but has been unable to get any 
positive staining. The only staining she's gotten is background. These are 
paraffin sections. The companies she bought the antibodies from has not 
been helpful. She's stained with Collagen type II & III and had no trouble 
with these. Any suggestions would be helpful.

Thank you,
Rita Angel, HT
University of Cincinnati