[Histonet] pressure cooking

[Katri Tuomala]

Hi Inga,
Sounds like your antibody needs heat retrieval to work on formalin fixed
tissue. It really does not matter how you create the heat. Generally
speaking, the lower the temperature, the longer the time in the heated
buffer. You will have to experiment, but microwave should work fine. The
problem with microwave oven is the possible hot spots, therefore giving you
uneven staining. Always use the same amount of buffer, the same number of
slides and  rotating base, if you have one.
Good luck!
Katri

Katri Tuomala
St.Joseph's Healthcare
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Inga Hansson" <Inga.Hansson <@t> neuro.uu.se>
To: <histonet <@t> pathology.swmed.edu>
Sent: Thursday, October 30, 2003 9:09 AM
Subject: [Histonet] pressure cooking


> Hi all!
>
> I have an antibody that requires pressure cooking for 1 minute (after
> having reached temperature) but I have no pressure cooker. Is it
> possible to heat in microwave-owen instead and for how long?
>
> Inga
>
> -- 
>
> Inga Hansson
> Dept. neuroscience,
> div. neurobiology
> PO Box 587, BMC
> Husargatan 3
> S-751 23 Uppsala
> SWEDEN
>
> Phone: +46(18) 471 4384
> Fax:   +46(18)559017
>
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